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KMID : 0379119930210040310
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Korean Journal of Mycology
1993 Volume.21 No. 4 p.310 ~ p.315
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Production of Ligninase in Agitated Submerged Cultures of Phanerochaete chrysosporium Diffuse Mycelia
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김경수/Kim, Kyung Soo
김영호/차동열/강안석/유창현/Suki C. Croan/Kim, Young Ho/Cha, Dong Yeul/Kang, An Seok/You, Chang Hyun
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Abstract
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Phanerochaete chrysosporium is a white rot fungus which secrets a family of lignindegrading enzymes under nutrient limitation. Ligninase was extracellularly produced in agitated submerged cultures of P. chrysosporium, SC 26. Addition of veratryl alcohol(4 mM), and benzyl alcohol(10 mM) with 0.1% Tween 20 to the culture medium stimulated ligninase production. However, figninase was not detected when both treatments of veratryl alcohol and benzyl alcohol without Tween 20 were added to the medium. Addition of 0.1% Tween 20 to the culture medium had little effect on ligninase activity. The ligninase activity was maximum on day 5-8 for veratryl alcohol, and benzyl alcohol with 0.1% Tween 20 additive medium.
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